In the Batman film series, the character of Harley Quinn, also known as Dr. Harleen Quinzel, has been portrayed by different actresses in various adaptations. Here are the actresses who have played Harley Quinn in live-action Batman films:
Margot Robbie: Margot Robbie portrayed Harley Quinn in the DC Extended Universe (DCEU) films. She first appeared as Harley Quinn in "Suicide Squad" (2016), which is set in the same universe as the Batman films. Margot Robbie reprised her role as Harley Quinn in "Birds of Prey" (2020) and "The Suicide Squad" (2021).
Arleen Sorkin: Arleen Sorkin provided the voice and physical inspiration for Harley Quinn in the animated Batman series, "Batman: The Animated Series" (1992-1995). Although not a live-action portrayal, her performance was influential in establishing the character's popularity.