Photosynthesis is a complex biochemical process that occurs in the chloroplasts of plant cells. It can be divided into two main stages: the light-dependent reactions and the light-independent reactions (Calvin cycle). Here's a brief overview of how photosynthesis works:
1. **Light-Dependent Reactions:**
- These reactions occur in the thylakoid membranes of chloroplasts.
- Light energy is absorbed by chlorophyll and other pigments in the thylakoid membranes, exciting electrons to higher energy levels.
- These energized electrons are passed along a series of proteins known as the electron transport chain (ETC), releasing energy in the process.
- This energy is used to pump hydrogen ions (H⁺) from the stroma into the thylakoid space, creating a concentration gradient.
- Meanwhile, water molecules are split by an enzyme called photolysis, releasing electrons, protons (H⁺ ions), and oxygen gas (O₂) as byproducts.
- The electrons from photolysis replace the ones lost by chlorophyll, and oxygen is released as a waste product.
- The energized electrons from the ETC are eventually transferred to NADP⁺, forming NADPH, a molecule that carries high-energy electrons.
2. **Light-Independent Reactions (Calvin Cycle):**
- These reactions take place in the stroma of chloroplasts.
- The Calvin cycle uses the ATP and NADPH produced in the light-dependent reactions to convert carbon dioxide (CO₂) into glucose.
- Carbon dioxide molecules are combined with a five-carbon compound called ribulose bisphosphate (RuBP) in a process called carbon fixation, facilitated by the enzyme rubisco.
- This results in the formation of an unstable six-carbon molecule, which immediately splits into two molecules of 3-phosphoglycerate (3-PGA).
- ATP and NADPH from the light-dependent reactions are used to convert 3-PGA into a three-carbon sugar called glyceraldehyde-3-phosphate (G3P).
- Some of the G3P molecules are used to regenerate RuBP to keep the Calvin cycle going, while others are used to produce glucose and other organic compounds.
Overall, photosynthesis is a crucial process that converts light energy into chemical energy stored in glucose, providing energy and sustenance for plants and ultimately supporting life on Earth.